| First Author | Duncan RR | Year | 1999 |
| Journal | J Biol Chem | Volume | 274 |
| Issue | 39 | Pages | 27347-50 |
| PubMed ID | 10488064 | Mgi Jnum | J:57874 |
| Mgi Id | MGI:1345903 | Doi | 10.1074/jbc.274.39.27347 |
| Citation | Duncan RR, et al. (1999) Transient, phorbol ester-induced DOC2-Munc13 interactions in vivo [published erratum appears in J Biol Chem 2000 Jan 21;275(3):2246]. J Biol Chem 274(39):27347-50 |
| abstractText | Munc13-1 and DOC2 have been implicated in the regulation of exocytosis. Here we demonstrate in vivo that these two proteins undergo a transient phorbol ester-mediated and protein kinase C-independent interaction, resulting in the translocation of DOC2 from a vesicular localization to the plasma membrane. The translocation of DOC2 is dependent upon the DOC2 Munc interacting domain that binds specifically to Munc13-1, whereas the association of DOC2 with intracellular membranes is dependent on its C2 domains. This is the first direct in vivo demonstration of a protein-protein interaction between two presynaptic proteins and may represent a molecular basis for phorbol ester-dependent enhancement of exocytosis. |
