| First Author | Yang X | Year | 2005 |
| Journal | J Biol Chem | Volume | 280 |
| Issue | 19 | Pages | 18710-6 |
| PubMed ID | 15778499 | Mgi Jnum | J:99923 |
| Mgi Id | MGI:3584225 | Doi | 10.1074/jbc.M500859200 |
| Citation | Yang X, et al. (2005) Mint represses transactivation of the type II collagen gene enhancer through interaction with alpha A-crystallin-binding protein 1. J Biol Chem 280(19):18710-6 |
| abstractText | Collagen type II is an extracellular matrix protein important for cartilage and bone formation, and its expression is controlled by multiple cis- and trans-acting elements, including the zinc finger transcription factor alpha A-crystallin-binding protein 1 (CRYBP1). Here we show that MSX2-interacting nuclear target protein (MINT), a conserved transcriptional repressor, associates with CRYBP1 and negatively regulates the transactivation of the collagen type II gene (Col2a1) enhancer. We identified CRYBP1 as a binding partner of MINT by screening a mouse embryonic cDNA library using the yeast two-hybrid system. We demonstrated that the C terminus of MINT interacts with the C terminus of CRYBP1 using the mammalian cell two-hybrid assay, glutathione S-transferase pull-down, and co-immunoprecipitation analyses. Furthermore, MINT and CRYBP1 form a complex on the Col2a1 enhancer, as shown by chromatin immunoprecipitation and gel shift assays. In the presence of CRYBP1, overexpression of MINT or its C-terminal fragment in cells repressed a reporter construct driven by the Col2a1 enhancer elements. This transcription repression is dependent on histone deacetylase, the main co-repressor recruited by MINT. The present study shows that MINT is involved in CRYBP1-mediated Col2a1 gene repression and may play a role in regulation of cartilage development. |
