| First Author | Moores TS | Year | 2005 |
| Journal | Brain Res | Volume | 1034 |
| Issue | 1-2 | Pages | 40-50 |
| PubMed ID | 15713258 | Mgi Jnum | J:96213 |
| Mgi Id | MGI:3529714 | Doi | 10.1016/j.brainres.2004.12.001 |
| Citation | Moores TS, et al. (2005) Properties of presynaptic P2X(7)-like receptors at the neuromuscular junction. Brain Res 1034(1-2):40-50 |
| abstractText | Adenosine triphosphate is released into the synaptic cleft of the neuromuscular junction during normal synaptic transmission, and in much greater quantities following injury and ischaemia. There is much data to suggest roles for presynaptic P2 receptors but little to demonstrate which specific receptor subunits are present. Here we show P2X(7) receptor subunits on presynaptic motor nerve terminals from birth, but no evidence for P2X(1), P2X(2), P2X(3), P2X(4), P2X(5) or P2X(6) receptor subunits. Further, P2X receptor subunits are present as multimeric, membrane-inserted receptors. A selective agonist, 2'-3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP: 100 muM), triggers vesicle release from motor nerve terminals, which is blocked by P2X(7)RS-specific concentrations of periodate oxidised ATP (OxATP: 100 muM) and brilliant blue G (BBG: 1 muM), but not by suramin (100 muM). Vesicle release is enhanced in the absence of extracellular divalent cations and occurs through activation of the ion channel and not any associated large pore, as we failed to label nerve terminals with large membrane-impermeant molecules after addition of BzATP. We conclude that a P2X(7)-like receptor is present at mouse motor nerve terminals, and that their activation promotes vesicle release. |
