| First Author | Papp L | Year | 2007 |
| Journal | Biochem Biophys Res Commun | Volume | 355 |
| Issue | 2 | Pages | 568-74 |
| PubMed ID | 17306762 | Mgi Jnum | J:118643 |
| Mgi Id | MGI:3700006 | Doi | 10.1016/j.bbrc.2007.02.014 |
| Citation | Papp L, et al. (2007) P2X7 receptor mediated phosphorylation of p38MAP kinase in the hippocampus. Biochem Biophys Res Commun 355(2):568-74 |
| abstractText | This study was designed to explore the effect of P2X7 receptor (P2X7R) activation on the expression of p38 MAP kinase (p38 MAPK) enzyme in hippocampal slices of wild-type (WT) and P2X7R(-/-) mice using the Western blot technique and to clarify its role in P2X7 receptor mediated [(3)H]glutamate release. ATP (1mM) and the P2X7R agonist BzATP (100muM) significantly increased p38 MAPK phosphorylation in WT mice, and these effects were absent in the hippocampal slices of P2X7R(-/-) mice. Both ATP- and BzATP-induced p38 MAPK phosphorylations were sensitive to the p38 MAP kinase inhibitor, SB203580 (1muM). ATP elicited [(3)H]glutamate release from hippocampal slices, which was significantly attenuated by SB203580 (1muM) but not by the extracellular signal-regulated kinase (ERK1/2) inhibitor, PD098095 (10muM). Consequently, we suggest that P2X7Rs and p38 MAPK are involved in the stimulatory effect of ATP on glutamate release in the hippocampal slices of WT mice. |
