| First Author | Garcia GG | Year | 2003 |
| Journal | Eur J Immunol | Volume | 33 |
| Issue | 12 | Pages | 3464-72 |
| PubMed ID | 14635057 | Mgi Jnum | J:90603 |
| Mgi Id | MGI:3044274 | Doi | 10.1002/eji.200324310 |
| Citation | Garcia GG, et al. (2003) Age-related defects in CD4+ T cell activation reversed by glycoprotein endopeptidase. Eur J Immunol 33(12):3464-72 |
| abstractText | CD4(+) T cells from old mice show defects in the activation process including deficiency in the formation of immunosynapses with antigen-presenting cells. We show that CD4(+) T cells from old mice express unusually high levels of glycosylated forms of the bulky T cell glycoprotein CD43, particularly on a subset of functionally anergic cells expressing P-glycoprotein. T cells from old donors also show a decline in the association of CD43 with cytoskeletal matrix and in the proportion of T cells that can exclude CD43 from the synapse. O-sialoglycoprotein endopeptidase, which removes the external domain of CD43 and other O-sialoglycoproteins from the aged naive CD4(+) T cells of TCR-transgenic mice, restores early agonist-independent stages and later agonist-dependent stages of synapse formation as well as expression of the activation markers CD69 and CD25 to the levels found in the young mice. These data support a model in which O-glycosylated forms of T cell surface molecules, including CD43, are largely responsible for age-related defects in TCR signaling and function. |
