| First Author | Blencowe BJ | Year | 1998 |
| Journal | Genes Dev | Volume | 12 |
| Issue | 7 | Pages | 996-1009 |
| PubMed ID | 9531537 | Mgi Jnum | J:70954 |
| Mgi Id | MGI:2148694 | Doi | 10.1101/gad.12.7.996 |
| Citation | Blencowe BJ, et al. (1998) A coactivator of pre-mRNA splicing. Genes Dev 12(7):996-1009 |
| abstractText | The nuclear matrix antigen recognized by the monoclonal antibody (mAb) B1C8 is a novel serine (S) and arginine (R)-rich protein associated with splicing complexes and is named here SRm160 (SR-related matrix protein of 160 kD). SRm160 contains multiple SR repeats, but unlike proteins of the SR family of splicing factors, lacks an RNA recognition motif. SRm160 and a related protein SRm300 (the 300-kD nuclear matrix antigen recognized by mAb B4A11) form a complex that is required for the splicing of specific pre-mRNAs. The SRm160/300 complex associates with splicing complexes and promotes splicing through interactions with SR family proteins. Binding of SRm160/300 to pre-mRNA is normally also dependent on U1 snRNP and is stabilized by U2 snRNP. Thus, SRm160/300 forms multiple interactions with components bound directly to important sites within pre-mRNA. The results suggest that a complex of the nuclear matrix proteins SRm160 and SRm300 functions as a coactivator of pre-mRNA splicing. |
