| First Author | Aghajanian S | Year | 2002 |
| Journal | Biochem J | Volume | 365 |
| Issue | Pt 1 | Pages | 13-8 |
| PubMed ID | 11994049 | Mgi Jnum | J:88247 |
| Mgi Id | MGI:3029834 | Doi | 10.1042/BJ20020569 |
| Citation | Aghajanian S, et al. (2002) Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase). Biochem J 365(Pt 1):13-8 |
| abstractText | The final two enzymes in the CoA biosynthetic pathway, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), are separate proteins in prokaryotes, but exist as a bifunctional enzyme in pig liver. In the present study we have obtained sequence information from purified pig-liver enzyme, and identified the corresponding cDNA in a number of species. The human gene localizes to chromosome 17q12-21 and contains regions with sequence similarity to the monofunctional Escherichia coli DPCK and PPAT. The recombinant 564-amino-acid human protein confirmed the associated transferase and kinase activities, and gave similar kinetic properties to the wild-type pig enzyme. |
