| First Author | Padmakumar VC | Year | 2005 |
| Journal | J Cell Sci | Volume | 118 |
| Issue | Pt 15 | Pages | 3419-30 |
| PubMed ID | 16079285 | Mgi Jnum | J:100177 |
| Mgi Id | MGI:3587451 | Doi | 10.1242/jcs.02471 |
| Citation | Padmakumar VC, et al. (2005) The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope. J Cell Sci 118(Pt 15):3419-30 |
| abstractText | Nesprins form a novel class of nuclear envelope-anchored spectrin-repeat proteins. We show that a direct association of their highly conserved C-terminal luminal domain with the inner nuclear membrane protein Sun1 mediates their nuclear envelope localisation. In Nesprin-1 and Nesprin-2 the conserved C-terminal amino acids PPPX are essential for the interaction with a C-terminal region in Sun1. In fact, Sun1 is required for the proper nuclear envelope localisation of Nesprin-2 as shown using dominant-negative mutants and by knockdown of Sun1 expression. Sun1 itself does not require functional A-type lamins for its localisation at the inner nuclear membrane in mammalian cells. Our findings propose a conserved nuclear anchorage mechanism between Caenorhabditis elegans and mammals and suggest a model in which Sun1 serves as a ;structural bridge' connecting the nuclear interior with the actin cytoskeleton. |
