| First Author | Yokohari K | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 289 |
| Issue | 2 | Pages | 414-20 |
| PubMed ID | 11716489 | Mgi Jnum | J:73112 |
| Mgi Id | MGI:2154586 | Doi | 10.1006/bbrc.2001.6008 |
| Citation | Yokohari K, et al. (2001) Isoform-Dependent Interaction of BRDG1 with Tec Kinase. Biochem Biophys Res Commun 289(2):414-20 |
| abstractText | Tec is the prototype of an emerging family of protein-tyrosine kinases. Tec and Btk, another member of this family, together participate in the development of B-cell immune system. We previously identified one of the downstream messengers for human Tec kinase, BRDG1. BRDG1 is associated with Tec and becomes tyrosine-phosphorylated in B-cells by the engagement of B-cell antigen receptor (BCR). Here we show that overexpression of BRDG1 strongly augments BCR-mediated activation of cAMP-response element binding protein (CREB) but not that of c-Jun and the promoters of c-MYC and BCL-xL genes. Furthermore, we isolated the murine orthologue of BRDG1. Three isoforms of BRDG1 are generated by alternative splicing of the message. Two of them have a deletion of 33 amino acids in a Pleckstrin homology (PH) domain of BRDG1. Both the tyrosine-phosphorylation and CREB-activating ability of BRDG1 were isoform-dependent, suggesting a role of the PH domain of BRDG1. These data have identified a novel regulatory mechanism of CREB family of transcriptional factors. (c)2001 Elsevier Science. |
