| First Author | Jakab G | Year | 1994 |
| Journal | Brain Res Dev Brain Res | Volume | 80 |
| Issue | 1-2 | Pages | 290-4 |
| PubMed ID | 7955356 | Mgi Jnum | J:19750 |
| Mgi Id | MGI:67883 | Doi | 10.1016/0165-3806(94)90116-3 |
| Citation | Jakab G, et al. (1994) The calcitonin gene-related peptide (CGRP) phenotype is expressed early and up-regulated by resiniferatoxin (RTX) in mouse sensory neurons. Brain Res Dev Brain Res 80(1-2):290-4 |
| abstractText | Calcitonin gene-related peptide (CGRP) immunoreactivity was detected at day 2 in vitro (embryonic day 15) in developing mouse dorsal root ganglion (DRG) neurons in primary culture. During 2 weeks of culture the proportion of CGRP-immunoreactive (CGRP-IR) neurons remained around 65-70%, much higher than usually found in adult animals (45-50%). Treatment of cultures with the capsaicin analog resiniferatoxin (RTX; 0.3-30 nM) significantly augmented CGRP immunoreactivity per neuron at all ages investigated without increasing the number of CGRP-immunoreactive cells. The increased CGRP immunoreactivity was observed both in the axonal varicosities and in the perinuclear region of cell bodies. This RTX-induced increase in CGRP immunoreactivity was completely blocked by Ruthenium red (RR). Treatment with the non-esterified form of RTX (resiniferol 9, 13, 14 orthophenylacetate, ROPA) produced no increase. These results suggest that: (1) early expression of the CGRP phenotype is regulated in a cell-autonomous way in developing mouse DRG neurons in vitro; and (2) the RTX-induced increase in CGRP biosynthesis is most likely the result of activating the capsaicin/RTX receptor rather than directly activating the protein kinase C (PKC) pathway in vitro. The results may also reflect qualitative and quantitative differences in capsaicin/RTX sensitivity of sensory neurons between embryonal and adult ages. |
