| First Author | Gu Y | Year | 1990 |
| Journal | Neuron | Volume | 5 |
| Issue | 2 | Pages | 147-57 |
| PubMed ID | 2383398 | Mgi Jnum | J:19812 |
| Mgi Id | MGI:67940 | Doi | 10.1016/0896-6273(90)90305-y |
| Citation | Gu Y, et al. (1990) Properties of embryonic and adult muscle acetylcholine receptors transiently expressed in COS cells. Neuron 5(2):147-57 |
| abstractText | We used transient transfection in COS cells to compare the properties of mouse muscle acetylcholine receptors (AChRs) containing alpha, beta, delta, and either gamma or epsilon subunits. gamma- and epsilon-AChRs had identical association rates for binding 125I-alpha-bungarotoxin, and identical curves for inhibition of toxin binding by d-tubocurarine, but epsilon-AChRs had a significantly longer half-time of turnover in the membrane than gamma-AChRs. A myasthenic serum specific for the embryonic form of the AChR reduced toxin binding to gamma-, but not epsilon-AChRs. The gamma-AChRs had channel characteristics of embryonic AChRs, whereas the major class of epsilon-AChR channels had the characteristics of adult AChRs. Two minor channel classes with smaller conductances were also seen with epsilon-AChR. Thus, some, but not all, of the differences between AChRs at adult endplates and those in the extrasynaptic membrane can be explained by the difference in subunit composition of gamma- and epsilon-AChRs. |
