| First Author | Walker G | Year | 1989 |
| Journal | J Biol Chem | Volume | 264 |
| Issue | 1 | Pages | 496-500 |
| PubMed ID | 2909534 | Mgi Jnum | J:88666 |
| Mgi Id | MGI:3036595 | Doi | 10.1016/S0021-9258(17)31285-1 |
| Citation | Walker G, et al. (1989) The role of caldesmon in the regulation of receptor capping in mouse T-lymphoma cell. J Biol Chem 264(1):496-500 |
| abstractText | Several complementary techniques, including immunocytochemical and immunobiochemical analyses, two-dimensional gel electrophoresis, and peptide mapping, were used in this study to examine the involvement of caldesmon in lymphocyte receptor capping. We have found a lymphoma 140-kDa polypeptide that is structurally similar to muscle caldesmon, suggesting that this polypeptide may be a lymphoma caldesmon. When lymphoma 140-kDa polypeptide is extracted from permeabilized cells using 25 mM MgCl2, capping is inhibited. Adding the 140-kDa protein or gizzard caldesmon back to the extracted cells restores their ability to cap. These findings suggest that actin-linked regulatory proteins such as caldesmon may be critically important to actomyosin-mediated contraction which, in turn, is responsible for collecting receptors into cap structures. |
