| First Author | Takahashi S | Year | 2003 |
| Journal | J Biol Chem | Volume | 278 |
| Issue | 49 | Pages | 49129-33 |
| PubMed ID | 14551201 | Mgi Jnum | J:86779 |
| Mgi Id | MGI:2682086 | Doi | 10.1074/jbc.C300289200 |
| Citation | Takahashi S, et al. (2003) Role for Fes/Fps tyrosine kinase in microtubule nucleation through is Fes/CIP4 homology domain. J Biol Chem 278(49):49129-33 |
| abstractText | We have previously demonstrated that Fes/Fps (Fes) tyrosine kinase is involved in Semaphorin3A-mediated signaling. Here we report a role for Fes tyrosine kinase in microtubule dynamics. A fibrous formation of Fes was observed in a kinase-dependent manner, which associated with microtubules and functionally correlated with microtubule bundling. Microtubule regeneration assays revealed that Fes aggregates colocalized with gamma-tubulin at microtubule nucleation sites in a Fes/CIP4 homology (FCH) domain-dependent manner and that expression of FCH domain-deleted Fes mutants blocked normal centrosome formation. In support of these observations, mouse embryonic fibroblasts derived from Fes-deficient mice displayed an aberrant structure of nucleation and centrosome with unbundling and disoriented filaments of microtubules. Our findings suggest that Fes plays a critical role in microtubule dynamics including microtubule nucleation and bundling through its FCH domain. |
