| First Author | Crossley M | Year | 1994 |
| Journal | J Biol Chem | Volume | 269 |
| Issue | 22 | Pages | 15440-4 |
| PubMed ID | 8195185 | Mgi Jnum | J:18481 |
| Mgi Id | MGI:66763 | Doi | 10.1016/s0021-9258(17)40698-3 |
| Citation | Crossley M, et al. (1994) Regulation of the erythroid Kruppel-like factor (EKLF) gene promoter by the erythroid transcription factor GATA-1. J Biol Chem 269(22):15440-4 |
| abstractText | Erythroid Kruppel-like factor (EKLF) is an erythroid-specific transcription factor that binds a CACCC motif found in the human beta-globin gene promoter. We have studied the promoter of the EKLF gene and identified binding sites for the transcription factors GATA-1 and CCAAT-binding Protein 1 (CP1). We show that both types of binding sites are required for full activity, and that the GATA motif at -60 is essential. The EKLF promoter can be directly activated in nonerythroid cells in cotransfection experiments by forced expression of GATA-1. These results suggest that EKLF is dependent on GATA-1 for its expression and lies downstream of, or coincident with, GATA-1 in a regulatory hierarchy in erythroid development. |
