| First Author | Zhou D | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1425 |
| Issue | 1 | Pages | 204-8 |
| PubMed ID | 9813328 | Mgi Jnum | J:50261 |
| Mgi Id | MGI:1290117 | Doi | 10.1016/s0304-4165(98)00070-1 |
| Citation | Zhou D, et al. (1998) Expression of beta1,4-galactosyltransferase in the development of mouse brain. Biochim Biophys Acta 1425(1):204-8 |
| abstractText | Beta1,4-galactosyltransferase (GalTase, EC 2.4.1.38) transfers galactose to the terminal N-acetylglucosamine of complex-type N-glycans, which have great importance for cell-cell interactions during fertilization and early embryogenesis. In this study, the activity of beta1,4-galactosyltransfer-ase in mouse brain during development was measured with the method of reverse HPLC using a fluorescence-labeled biantenary sugar chain, GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Man alpha1-3) Manbeta1-4GlcNAcbeta1-4GlcNAc-PA. The level of messenger RNA of this enzyme during the development of mouse brain was also investigated with Northern blot analysis. The results showed that: (1)beta1,4-galactosyltransferase showed similar branch specificity and kinetics for the biantenary substrate during development; (2) GalTase activity in fetal mouse brain was four times higher than that in adult mouse brain and decreased gradually in the course of development; (3) messenger RNA level was highest in fetal mouse and decreased dramatically after birth. However, the contents of mRNA were not parallel to the enzyme activity. |
