| First Author | Li S | Year | 2007 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 48 |
| Issue | 7 | Pages | 3292-300 |
| PubMed ID | 17591901 | Mgi Jnum | J:123324 |
| Mgi Id | MGI:3717988 | Doi | 10.1167/iovs.06-1482 |
| Citation | Li S, et al. (2007) The requirement of pax6 for postnatal eye development: evidence from experimental mouse chimeras. Invest Ophthalmol Vis Sci 48(7):3292-300 |
| abstractText | PURPOSE: The small eye mouse mutant (Sey) is caused by a mutation of the Pax6 gene. Previous studies, in which aggregation chimeras were used, have demonstrated that Sey/Sey cells contribute poorly to the neural retina forming small clumps of cells restricted to the inner retina at embryonic day 16.5. In addition, Sey/+ cells are absent from the lens epithelium during this embryonic period and postnatally. This study was conducted to determine the fates of these Sey/Sey and Sey/+ cells with continued development in chimeric mouse eyes. METHODS: Observations were made on heterozygous and homozygous Sey cells in chimeric eyes from postnatal day (P)0 to P10. RESULTS: In Sey/Sey<-->wild-type (wt) chimeras, all Sey/Sey cells originating from retinal progenitor cells died at perinatal times. The only remaining Sey/Sey cells in the neural retina were associated with blood vessels, including vascular endothelial cells, pericytes, astrocytes, and microglia, which have extraretinal origins. In contrast, Sey/+ cells formed all retinal cell classes. As previously reported, Sey/Sey cells were absent from the lens and corneal epithelium. However, in contrast to previous reports, Sey/+ cells contributed to the lens epithelium as well as corneal tissues, and Sey/Sey cells were absent from the anterior retinal pigment epithelium. CONCLUSIONS: All evidence showed that, when Pax6 is absent at the initial stages of the development, Sey/Sey cells that contribute to the neural retina die, even when wild-type cells are available to provide normal environmental cues. |
