| First Author | Leco KJ | Year | 1997 |
| Journal | Dev Genet | Volume | 21 |
| Issue | 1 | Pages | 55-60 |
| PubMed ID | 9291580 | Mgi Jnum | J:42729 |
| Mgi Id | MGI:1096214 | Doi | 10.1002/(SICI)1520-6408(1997)21:1<55::AID-DVG6>3.0.CO;2-7 |
| Citation | Leco KJ, et al. (1997) Matrix metalloproteinase-9 maps to the distal end of chromosome 2 in the mouse. Dev Genet 21(1):55-60 |
| abstractText | The activity and expression of matrix metalloproteinase-9/gelatinase B (MMP-9), an enzyme implicated in the implantation process in mice, was investigated in normal and parthenogenetic blastocyst outgrowths. Conditioned media from parthenogenetic blastocysts after 4 days of culture had reduced levels of MMP-9 activity compared to conditioned medium from normal outgrowths. Levels of MMP-9 mRNA assayed by reverse transcription-polymerase chain reaction methods were also reduced in parthenogenetic blastocysts compared to normal outgrowths. Genetic mapping studies showed that Mmp9 maps to the distal end of chromosome 2 near the proximal boundary of a region affected by genomic imprinting. Both parental alleles of Mmp9, however, are expressed in 11.5-day embryos derived from interspecific crosses of Mus musculus and Mus spretus. Thus, loss of MMP-9 activity in parthenogenetic blastocysts does not appear to be due to imprinting but, rather, due to a defect of trophoblast giant cell proliferation and differentiation. |
