| First Author | Ashley PL | Year | 1986 |
| Journal | Am J Hum Genet | Volume | 39 |
| Pages | A186 (Abstr.) | Mgi Jnum | J:4853 |
| Mgi Id | MGI:53335 | Citation | Ashley PL, et al. (1986) Identification of novel phosphofructokinase loci in mouse and man. Am J Hum Genet 39:A186 (Abstr.) |
| abstractText | Full text of Abstract. A186 Molecular Genetics. (551) 1.83. IDENTIFICATION OF NOVEL PHOSPHOFRUCTOKINASE LOCI IN MOUSE AND MAN. P.L. Ashley, R.R. Flandermeyer, D.R. Cox, Univ. of California, San Francisco. As a first step in the study of murine and human phosphofructokinase (PFK) gene families, we have isolated and characterized a mouse PFK cDNA clone. DNA sequence analysis of the 2.6 kb cDNA insert revealed 85% amino acid homology with rabbit muscle PFK and indicated that the insert represents the majority of a PFK mRNA, including the region from codon 75 through the 3' untranslated region. We have mapped this PFK gene to mouse chromosome 15 using a panel of mouse x hamster somatic cell hybrids. Northern blot analysis of adult mouse RNAs reveals high levels of this PFK mRNA in brain and testes, with less abundant expression in kidney, spleen, and liver; the liver mRNA being barely detectable, and the testes mRNA being slightly larger than those in the other tissues. This pattern of expression differs from that of previously described mouse PFK subunits, suggesting that we have identified a gene encoding a novel mouse PFK. At high stringency our mouse PFK probe hybridizes with a single fragment on Southern blots of human genomic DNA. Using spot blot hybridization of sorted human chromosomes in conjunction with mouse x human somatic cell hybrids, we have assigned the cross-reacting DNA to chromosome 12. Since previously identified human PFK loci have been mapped to chromosomes 1 (PFKM), 10 (PFKP) and 21 (PFKL), these data provide evidence for a fourth PFK locus. |
