| First Author | Uesaka N | Year | 2014 |
| Journal | Science | Volume | 344 |
| Issue | 6187 | Pages | 1020-3 |
| PubMed ID | 24831527 | Mgi Jnum | J:211258 |
| Mgi Id | MGI:5574372 | Doi | 10.1126/science.1252514 |
| Citation | Uesaka N, et al. (2014) Retrograde semaphorin signaling regulates synapse elimination in the developing mouse brain. Science 344(6187):1020-3 |
| abstractText | Neural circuits are shaped by elimination of early-formed redundant synapses during postnatal development. Retrograde signaling from postsynaptic cells regulates synapse elimination. In this work, we identified semaphorins, a family of versatile cell recognition molecules, as retrograde signals for elimination of redundant climbing fiber to Purkinje cell synapses in developing mouse cerebellum. Knockdown of Sema3A, a secreted semaphorin, in Purkinje cells or its receptor in climbing fibers accelerated synapse elimination during postnatal day 8 (P8) to P18. Conversely, knockdown of Sema7A, a membrane-anchored semaphorin, in Purkinje cells or either of its two receptors in climbing fibers impaired synapse elimination after P15. The effect of Sema7A involves signaling by metabotropic glutamate receptor 1, a canonical pathway for climbing fiber synapse elimination. These findings define how semaphorins retrogradely regulate multiple processes of synapse elimination. |
