| First Author | Prudner BC | Year | 2014 |
| Journal | FEBS Lett | Volume | 588 |
| Issue | 24 | Pages | 4625-30 |
| PubMed ID | 25447537 | Mgi Jnum | J:216948 |
| Mgi Id | MGI:5610068 | Doi | 10.1016/j.febslet.2014.11.001 |
| Citation | Prudner BC, et al. (2014) alpha-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin. FEBS Lett 588(24):4625-30 |
| abstractText | alpha-Synemin contains a unique 312 amino acid insert near the end of its C-terminal tail. Therefore we set out to determine if the insert is a site of protein-protein interaction that regulates the sub-cellular localization of this large isoform of synemin. Yeast-two hybrid analysis indicated that this region is a binding site for the M10 region of titin. This was confirmed with GST pull-down assays. Co-immunoprecipitation of endogenous proteins indicated close association of the two proteins in vivo and immunostaining of cardiomyocytes demonstrated co-localization of the proteins at the M-band of the sarcomere. |
