| First Author | Phelan ML | Year | 1999 |
| Journal | Mol Cell | Volume | 3 |
| Issue | 2 | Pages | 247-53 |
| PubMed ID | 10078207 | Mgi Jnum | J:320039 |
| Mgi Id | MGI:6867271 | Doi | 10.1016/s1097-2765(00)80315-9 |
| Citation | Phelan ML, et al. (1999) Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Mol Cell 3(2):247-53 |
| abstractText | Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex. |
