| First Author | Itoh R | Year | 2013 |
| Journal | FEBS Lett | Volume | 587 |
| Issue | 14 | Pages | 2131-6 |
| PubMed ID | 23735699 | Mgi Jnum | J:199092 |
| Mgi Id | MGI:5500838 | Doi | 10.1016/j.febslet.2013.05.036 |
| Citation | Itoh R, et al. (2013) Imaging of heme/hemeproteins in nucleus of the living cells expressing heme-binding nuclear receptors. FEBS Lett 587(14):2131-6 |
| abstractText | Several factors involved in the core circadian rhythm are PAS domain proteins, one of which, neuronal PAS2 (NPAS2), contains a heme-binding motif. It is thought that heme controls the transcriptional activity of core circadian factors BMAL1-NPAS2, and that the heme-binding nuclear receptor REV-erbalpha negatively regulates the expression of BMAL1. To examine the role of heme in the nucleus, we expressed nuclear hemeproteins including the nuclear localization signal-added cytoglobin, NPAS2 and REV-erbalpha. Then, the living cells expressing these proteins were treated with 2',7'-dichlorodihydrofluorescin diacetate (DCFH-DA). The fluorescent signal derived from DCFH-DA was observed in the nucleus. When the cells were cultured with hemin, the signal of heme in the nucleus increased. Considering that DCFH-DA reacted with heme, we propose that the use of DCFH-DA could be useful in detection of the heme moiety of hemeprotein in vivo. |
