| First Author | Yurugi H | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 420 |
| Issue | 2 | Pages | 275-80 |
| PubMed ID | 22417827 | Mgi Jnum | J:219621 |
| Mgi Id | MGI:5621261 | Doi | 10.1016/j.bbrc.2012.02.149 |
| Citation | Yurugi H, et al. (2012) Expression of prohibitins on the surface of activated T cells. Biochem Biophys Res Commun 420(2):275-80 |
| abstractText | Prohibitins (prohibitin-1 and -2) comprise a family of highly conserved proteins that are mainly localized to mitochondria. Recent studies showed that prohibitins are up-regulated upon T cell activation and play an essential role in maintaining mitochondrial homeostasis. In the present study, we found that a considerable proportion of prohibitin-1 and -2 induced in response to T cell activation was expressed on the surface of activated T cells. When mouse and human T cells were stimulated with PMA and ionomycin, prohibitins expressed on the cell surface were increased significantly, peaking at 48 h after stimulation. Stimulation of mouse T cells with anti-CD3 and anti-CD28 antibodies also remarkably induced the cell surface expression of prohibitins. Their expression on the cell surface was also detected in T cell leukemia cells such as Jurkat cells. In Jurkat cells, prohibitin-1 and -2 were co-localized with CD3 on the cell surface, and anti-CD3 antibody-induced signaling, the MAP kinase cascade, was inhibited on treatment with protein A magnetic beads co-conjugated with anti-CD3 antibody and anti-prohibitin-1 or anti-prohibitin-2 antibody. These results suggest that prohibitins expressed on the surface of activated T cells are involved in the T cell receptor-mediated signaling cascade. |
