|  Help  |  About  |  Contact Us

Publication : PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein.

First Author  Lee H Year  2006
Journal  Genes Dev Volume  20
Issue  7 Pages  784-94
PubMed ID  16600910 Mgi Jnum  J:107295
Mgi Id  MGI:3620509 Doi  10.1101/gad.1392006
Citation  Lee H, et al. (2006) PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein. Genes Dev 20(7):784-94
abstractText  The mechanisms by which homeoproteins bind selectively to target genes in vivo have long remained unresolved. Here we report that PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein by regulating its subnuclear localization and proximity to target genes. We demonstrate that the interaction of Msx1 with PIAS1, but not its sumoylation, is required for Msx1 to function as an inhibitor of myoblast differentiation through repression of myogenic regulatory genes, such as MyoD. We find that PIAS1 enables Msx1 to bind selectively to a key regulatory element in MyoD, the CER, in myoblast cells and to distinguish the CER from other nonregulatory TAAT-containing sequences. We show that PIAS1 is required for the appropriate localization and retention of Msx1 at the nuclear periphery in myoblast cells. Furthermore, we demonstrate that myogenic regulatory genes that are repressed by Msx1, namely MyoD and Myf5, are located at the nuclear periphery in myoblast cells. We propose that a key regulatory event for DNA-binding specificity by homeoproteins in vivo is their appropriate targeting to subnuclear compartments where their target genes are located, which can be achieved by cofactors such as PIAS1.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

3 Bio Entities

Trail: Publication

0 Expression