| First Author | Oh J | Year | 2008 |
| Journal | J Biol Chem | Volume | 283 |
| Issue | 18 | Pages | 11924-34 |
| PubMed ID | 18303029 | Mgi Jnum | J:135538 |
| Mgi Id | MGI:3794005 | Doi | 10.1074/jbc.M705401200 |
| Citation | Oh J, et al. (2008) BAF60a interacts with p53 to recruit the SWI/SNF complex. J Biol Chem 283(18):11924-34 |
| abstractText | To understand the tumor-suppressing mechanism of the SWI/SNF chromatin remodeling complex, we investigated its molecular relationship with p53. Using the pREP4-luc episomal reporter, we first demonstrated that p53 utilizes the chromatin remodeling activity of the SWI/SNF complex to initiate transcription from the chromatin-structured promoter. Among the components of the SWI/SNF complex, we identified BAF60a as a mediator of the interaction with p53 by the yeast two-hybrid assay. p53 directly interacted only with BAF60a, but not with other components of the SWI/SNF complex, such as BRG1, SRG3, SNF5, or BAF57. We found out that multiple residues at the amino acid 108-150 region of BAF60a were involved in the interaction with the tetramerization domain of p53. The N-terminal fragment of BAF60a containing the p53-interacting region as well as small interfering RNA for baf60a inhibited the SWI/SNF complex-mediated transcriptional activity of p53. The uncoupling of p53 with the SWI/SNF complex resulted in the repression of both p53-dependent apoptosis and cell cycle arrest by the regulation of target genes. These results suggest that the SWI/SNF chromatin remodeling complex is involved in the suppression of tumors by the interaction with p53. |
