| First Author | Zhang Y | Year | 2021 |
| Journal | Biochem Biophys Res Commun | Volume | 545 |
| Pages | 105-111 | PubMed ID | 33548622 |
| Mgi Jnum | J:338226 | Mgi Id | MGI:6717323 |
| Doi | 10.1016/j.bbrc.2021.01.076 | Citation | Zhang Y, et al. (2021) Effects of transforming growth factor-beta1 on odontoblastic differentiation in dental papilla cells is determined by IPO7 expression level. Biochem Biophys Res Commun 545:105-111 |
| abstractText | Transforming growth factor beta1 (TGF-beta1) is one of the broad-spectrum growth-promoting factors that participate in tooth development. The influence of TGF-beta1 on the odontoblastic differentiation is still controvercy. Mouse primary dental papilla cells (mDPCs) as well as an immortalized mouse dental papilla cell line (mDPC6Ts) were treated with exogenous TGF-beta1 during odontoblastic differentiation. RT-qPCR, Western blot, alizarin red staining and ALP staining were carried out to investigate the influence of TGF-beta1 on odontoblastic differentiation. IPO7, important for SMAD complex translocation was also detected in mDPCs and mDPC6Ts in response to TGF-beta1. After silencing IPO7 by transfection, the translocation process of P-SMAD2 was investigated by nuclear and cytoplasmic extraction as well as co-immunoprecipitation assay. The odontogenic markers, mineralization and IPO7 expression were significantly up-regulated in TGF-beta1-treated mDPCs while down-regulated in mDPC6Ts. The total level of P-SMAD2 was not influenced by IPO7 in mDPCs, however, IPO7 could bind to P-SMAD2 and affect the nuclear-cytoplasm-shuttling of P-SMAD2. Our data demonstrated that TGF-beta1 plays opposite roles in odontoblast differentiation in mDPCs and immortalized mouse dental papilla cell line (mDPC6Ts), which is determined by IPO7. |
