| First Author | Tsukasaki M | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 410 |
| Issue | 4 | Pages | 766-70 |
| PubMed ID | 21689636 | Mgi Jnum | J:175244 |
| Mgi Id | MGI:5285024 | Doi | 10.1016/j.bbrc.2011.06.048 |
| Citation | Tsukasaki M, et al. (2011) Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-alpha. Biochem Biophys Res Commun 410(4):766-70 |
| abstractText | POEM, also known as nephronectin, is an extracellular matrix protein considered to be a positive regulator of osteoblast differentiation. In the present study, we found that tumor necrosis factor-alpha (TNF-alpha), a key regulator of bone matrix properties and composition that also inhibits terminal osteoblast differentiation, strongly inhibited POEM expression in the mouse osteoblastic cell line MC3T3-E1. TNF-alpha-induced down-regulation of POEM gene expression occurred in both time- and dose-dependent manners through the nuclear factor kappa B (NF-kappaB) pathway. In addition, expressions of marker genes in differentiated osteoblasts were down-regulated by TNF-alpha in a manner consistent with our findings for POEM, while over-expression of POEM recovered TNF-alpha-induced inhibition of osteoblast differentiation. These results suggest that TNF-alpha inhibits POEM expression through the NF-kappaB signaling pathway and down-regulation of POEM influences the inhibition of osteoblast differentiation by TNF-alpha. |
