| First Author | Rossier O | Year | 2012 |
| Journal | Nat Cell Biol | Volume | 14 |
| Issue | 10 | Pages | 1057-67 |
| PubMed ID | 23023225 | Mgi Jnum | J:193934 |
| Mgi Id | MGI:5469937 | Doi | 10.1038/ncb2588 |
| Citation | Rossier O, et al. (2012) Integrins beta1 and beta3 exhibit distinct dynamic nanoscale organizations inside focal adhesions. Nat Cell Biol 14(10):1057-67 |
| abstractText | Integrins in focal adhesions (FAs) mediate adhesion and force transmission to extracellular matrices essential for cell motility, proliferation and differentiation. Different fibronectin-binding integrins, simultaneously present in FAs, perform distinct functions. Yet, how integrin dynamics control biochemical and biomechanical processes in FAs is still elusive. Using single-protein tracking and super-resolution imaging we revealed the dynamic nano-organizations of integrins and talin inside FAs. Integrins reside in FAs through free-diffusion and immobilization cycles. Integrin activation promotes immobilization, stabilized in FAs by simultaneous connection to fibronectin and actin-binding proteins. Talin is recruited in FAs directly from the cytosol without membrane free-diffusion, restricting integrin immobilization to FAs. Immobilized beta3-integrins are enriched and stationary within FAs, whereas immobilized beta1-integrins are less enriched and exhibit rearward movements. Talin is enriched and mainly stationary, but also exhibited rearward movements in FAs, consistent with stable connections with both beta-integrins. Thus, differential transmission of actin motion to fibronectin occurs through specific integrins within FAs. |
