| First Author | Moujalled D | Year | 2011 |
| Journal | EMBO Rep | Volume | 12 |
| Issue | 1 | Pages | 77-83 |
| PubMed ID | 21151042 | Mgi Jnum | J:200504 |
| Mgi Id | MGI:5508733 | Doi | 10.1038/embor.2010.190 |
| Citation | Moujalled D, et al. (2011) Cyclic-AMP-dependent protein kinase A regulates apoptosis by stabilizing the BH3-only protein Bim. EMBO Rep 12(1):77-83 |
| abstractText | The proapoptotic Bcl2 homology domain 3(BH3)-only protein Bim is controlled by stringent post-translational regulation, predominantly through alterations in phosphorylation status. To identify new kinases involved in its regulation, we carried out a yeast two-hybrid screen using a non-spliceable variant of the predominant isoform--Bim(EL)--as the bait and identified the regulatory subunit of cyclic-AMP-dependent protein kinase A--PRKAR1A--as an interacting partner. We also show that protein kinase A (PKA) is a Bim(EL) isoform-specific kinase that promotes its stabilization. Inhibition of PKA or mutation of the PKA phosphorylation site within Bim(EL) resulted in its accelerated proteasome-dependent degradation. These results might have implications for human diseases that are characterized by abnormally increased PKA activity, such as the Carney complex and dilated cardiomyopathy. |
