| First Author | O'Toole CM | Year | 2000 |
| Journal | Mol Biol Cell | Volume | 11 |
| Issue | 5 | Pages | 1571-84 |
| PubMed ID | 10793136 | Mgi Jnum | J:169811 |
| Mgi Id | MGI:4942264 | Doi | 10.1091/mbc.11.5.1571 |
| Citation | O'Toole CM, et al. (2000) Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction. Mol Biol Cell 11(5):1571-84 |
| abstractText | Fertilization occurs after the completion of the sperm acrosome reaction, a secretory event that is triggered during gamete adhesion. ZP3, an egg zona pellucida glycoprotein, produces a sustained increase of the internal Ca(2+) concentration in mouse sperm, leading to acrosome reactions. Here we show that the sustained Ca(2+) concentration increase is due to the persistent activation of a Ca(2+) influx mechanism during the late stages of ZP3 signal transduction. These cells also possess a Ca(2+) store depletion-activated Ca(2+) entry pathway that is open after treatment with thapsigargin. Thapsigargin and ZP3 activate the same Ca(2+) permeation mechanism, as demonstrated by fluorescence quenching experiments and by channel antagonists. These studies show that ZP3 generates a sustained Ca(2+) influx through a store depletion-operated pathway and that this drives the exocytotic acrosome reaction. |
