| First Author | Wiggan O | Year | 1998 |
| Journal | Oncogene | Volume | 16 |
| Issue | 2 | Pages | 227-36 |
| PubMed ID | 9464541 | Mgi Jnum | J:45398 |
| Mgi Id | MGI:1195383 | Doi | 10.1038/sj.onc.1201534 |
| Citation | Wiggan O, et al. (1998) Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Oncogene 16(2):227-36 |
| abstractText | The specific loss of pRB or p107 together with p130 disrupts the normal development of only a very limited spectrum of tissues. These developmental defects have been attributed primarily to deregulation of E2F activity and consequent uncontrolled proliferation. We hypothesized, however, that the tissue-specific nature of these defects may also reflect deregulation of pRB-family associated factors that are specifically involved in determining cell fate. We report here that the pRB-family members interact with transcription factors which contain paired-like homeodomains such as MHox, Chx10 and Pax-3. The interaction between the pRB-family and the paired-like homeodomain proteins was initially identified in a yeast two-hybrid screen where the N-terminal portion of p130 was used to isolate interacting factors from an embryonic mouse library. This interaction was confirmed by in vitro binding and co-immunoprecipitation assays. We show further that co- expression of Pax-3 dependent pRB, p107 or p130 with Pax-3 causes repression of activated transcription from the c-met promoter. These data demonstrate that the pRB-family proteins can modulate the activity of factors which specifically control cell fate and/or differentiation as well as controlling cell cycle regulators. |
