| First Author | Bhoumik A | Year | 2005 |
| Journal | Mol Cell | Volume | 18 |
| Issue | 5 | Pages | 577-87 |
| PubMed ID | 15916964 | Mgi Jnum | J:240286 |
| Mgi Id | MGI:5882896 | Doi | 10.1016/j.molcel.2005.04.015 |
| Citation | Bhoumik A, et al. (2005) ATM-dependent phosphorylation of ATF2 is required for the DNA damage response. Mol Cell 18(5):577-87 |
| abstractText | Activating transcription factor 2 (ATF2) is regulated by JNK/p38 in response to stress. Here, we demonstrate that the protein kinase ATM phosphorylates ATF2 on serines 490 and 498 following ionizing radiation (IR). Phosphoantibodies to ATF2(490/8) reveal dose- and time-dependent phosphorylation of ATF2 by ATM that results in its rapid colocalization with gamma-H2AX and MRN components into IR-induced foci (IRIF). Inhibition of ATF2 expression decreased recruitment of Mre11 to IRIF, abrogated S phase checkpoint, reduced activation of ATM, Chk1, and Chk2, and impaired radioresistance. ATF2 requires neither JNK/p38 nor its DNA binding domain for recruitment to IRIF and the S phase checkpoint. Our findings identify a role for ATF2 in the DNA damage response that is uncoupled from its transcriptional activity. |
