| First Author | Konno R | Year | 1984 |
| Journal | J Nutr | Volume | 114 |
| Issue | 9 | Pages | 1617-21 |
| PubMed ID | 6147398 | Mgi Jnum | J:343411 |
| Mgi Id | MGI:7565918 | Doi | 10.1093/jn/114.9.1617 |
| Citation | Konno R, et al. (1984) Involvement of D-amino-acid oxidase in D-amino acid utilization in the mouse. J Nutr 114(9):1617-21 |
| abstractText | Mutant ddY/DAO- mice lacking D-amino-acid oxidase were examined for their ability to utilize D-phenylalanine in place of its L-isomer. Chemically defined milk powder devoid of phenylalanine was used as a basal diet. Adult ddY/DAO- mice and normal ddY/DAO+ mice having D-amino-acid oxidase lost weight every day when fed this diet. However, they maintained their weight when fed the milk powder diet supplemented with 0.33% L-phenylalanine. Although adult ddY/DAO+ mice maintained their weight when fed the milk powder diet supplemented with 0.33% D-phenylalanine, ddY/DAO- mice lost weight while feeding on this diet, which indicated that the ddY/DAO- mice could not utilize D-phenylalanine in place of its L-isomer. Both ddY/DAO- and ddY/DAO+ mice maintained their weight when feeding on the milk powder diet enriched with 0.41% sodium phenylpyruvate, which suggested to us that the ddY/DAO- mice had the ability to invert this alpha-keto acid to L-phenylalanine. Therefore, these results indicate that D-amino-acid oxidase is indispensable for D-amino acid utilization. This is consistent with the concept that D-amino acids are oxidatively deaminated to the corresponding alpha-keto acids by D-amino-acid oxidase and that these keto intermediates are then asymmetrically reaminated to their L-amino acids. |
