| First Author | Wasiak S | Year | 2003 |
| Journal | FEBS Lett | Volume | 555 |
| Issue | 3 | Pages | 437-42 |
| PubMed ID | 14675752 | Mgi Jnum | J:87183 |
| Mgi Id | MGI:2683832 | Doi | 10.1016/s0014-5793(03)01299-7 |
| Citation | Wasiak S, et al. (2003) Characterization of a gamma-adaptin ear-binding motif in enthoprotin. FEBS Lett 555(3):437-42 |
| abstractText | Enthoprotin, a newly identified component of clathrin-coated vesicles, interacts with the trans-Golgi network (TGN) clathrin adapters AP-1 and GGA2. Here we perform a multi-faceted analysis of the site in enthoprotin that is responsible for the binding to the gamma-adaptin ear (gamma-ear) domain of AP-1. Alanine scan mutagenesis and nuclear magnetic resonance (NMR) studies reveal the full extent of the site as well as critical residues for this interaction. NMR studies of the gamma-ear in complex with a synthetic peptide from enthoprotin provide structural details of the binding site for TGN accessory proteins within the gamma-ear. |
