| First Author | Yu F | Year | 2016 |
| Journal | Mol Med Rep | Volume | 13 |
| Issue | 1 | Pages | 895-900 |
| PubMed ID | 26648560 | Mgi Jnum | J:269549 |
| Mgi Id | MGI:6274954 | Doi | 10.3892/mmr.2015.4593 |
| Citation | Yu F, et al. (2016) Connexin43 knockdown in bone marrowderived dendritic cells by small interfering RNA leads to a diminished T-cell stimulation. Mol Med Rep 13(1):895-900 |
| abstractText | Dendritic cells, the most powerful type of antigenpresenting cells, have the unique ability to induce primary immune responses. Connexin43 expression is upregulated to increase gap junctions when immune cells are exposed to inflammatory factors. The present study applied smallinterfering RNA (siRNA) to decrease connexin43 expression. The results showed that silencing of connexin43 using siRNA resulted in arrest of bone marrowderived dendritic cell (BMDC) maturation as evidenced by reduced expression of major histocompatibility complex II, CD40, CD80 and CD86. Functionally, connexin43silenced BMDC showed a markedly decreased capability to induce T-cell stimulation. In conclusion, the present study demonstrated that antigens present on BMDCs can be suppressed by connexin43 knockdown in BMDCs. The present study therefore presented an effective method to modulate the immunology of BMDCs. |
