| First Author | Roder K | Year | 1999 |
| Journal | Gene | Volume | 234 |
| Issue | 1 | Pages | 61-9 |
| PubMed ID | 10393239 | Mgi Jnum | J:113849 |
| Mgi Id | MGI:3687730 | Doi | 10.1016/s0378-1119(99)00180-8 |
| Citation | Roder K, et al. (1999) Interaction between the two ubiquitously expressed transcription factors NF-Y and Sp1. Gene 234(1):61-9 |
| abstractText | The regulation of the rat fatty acid synthase gene by mediators such as diet, hormones, cAMP, sterols or retinoic acid is controlled by three NF-Y binding sites. All three sites have a neighbouring Sp1-binding GC-box. This NF-Y/Sp1 motif is conserved in the FAS promoters of rat, human, goose and chicken. We have previously shown cooperative binding of NF-Y and Sp1 to the promoter region at -500 coincident with a diet-induced DNAse I-hypersensitive site. Here, we show an in-vivo interaction of NF-YA with Sp1 using the yeast two-hybrid system. The interacting domains are located between amino acids 55 and 139 of the NF-Y subunit NF-YA and between amino acids 139 and 344 of Sp1. In addition, we show by co-immunoprecipitation direct interaction of NF-Y subunit NF-YA with Sp1 in extracts of rat hepatoma cells H4IIE. Furthermore, we demonstrate by the GST pull-down assay that NF-YA interacts physically with Sp1 in-vitro in the absence of DNA. Therefore, NF-Y can be added to the list of transcription factors interacting with Sp1. |
