| First Author | Hwang SS | Year | 2010 |
| Journal | Immunology | Volume | 131 |
| Issue | 1 | Pages | 50-8 |
| PubMed ID | 20636338 | Mgi Jnum | J:166393 |
| Mgi Id | MGI:4844227 | Doi | 10.1111/j.1365-2567.2010.03271.x |
| Citation | Hwang SS, et al. (2010) GATA-binding protein-3 regulates T helper type 2 cytokine and ifng loci through interaction with metastasis-associated protein 2. Immunology 131(1):50-8 |
| abstractText | GATA-binding protein-3 (GATA-3) regulates the T helper type 2 (Th2) cytokine locus through induction of chromatin remodelling. However, the molecular mechanism for this is poorly understood. To understand this mechanism better, we screened GATA-3 interacting proteins using affinity purification and mass spectrometry. We found that GATA-3 bound to metastasis-associated protein 2 (MTA-2), a component of the NuRD chromatin remodelling complex. GATA-3 and MTA-2 in turn bound to several regulatory regions of the Th2 cytokine locus and the ifng promoter. Cell transfection assay showed that MTA-2 acted as an antagonist with GATA-3 in the expression of Th2 cytokines, but co-operated with GATA-3 in the repression of the ifng gene expression. These results suggest that GATA-3 interacts with MTA-2 to co-ordinately regulate Th2 cytokine and ifng loci during T helper cell differentiation. |
