| First Author | Madarampalli B | Year | 2015 |
| Journal | Cell | Volume | 162 |
| Issue | 3 | Pages | 580-92 |
| PubMed ID | 26213385 | Mgi Jnum | J:224833 |
| Mgi Id | MGI:5689188 | Doi | 10.1016/j.cell.2015.06.055 |
| Citation | Madarampalli B, et al. (2015) ATF5 Connects the Pericentriolar Materials to the Proximal End of the Mother Centriole. Cell 162(3):580-92 |
| abstractText | Although it is known that the centrioles play instructive roles in pericentriolar material (PCM) assembly and that the PCM is essential for proper centriole formation, the mechanism that governs centriole-PCM interaction is poorly understood. Here, we show that ATF5 forms a characteristic 9-fold symmetrical ring structure in the inner layer of the PCM outfitting the proximal end of the mother centriole. ATF5 controls the centriole-PCM interaction in a cell-cycle- and centriole-age-dependent manner. Interaction of ATF5 with polyglutamylated tubulin (PGT) on the mother centriole and with PCNT in the PCM renders ATF5 as a required molecule in mother centriole-directed PCM accumulation and in PCM-dependent centriole formation. ATF5 depletion blocks PCM accumulation at the centrosome and causes fragmentation of centrioles, leading to the formation of multi-polar mitotic spindles and genomic instability. These data show that ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the PCM. |
