| First Author | Wu X | Year | 2008 |
| Journal | FEBS Lett | Volume | 582 |
| Issue | 15 | Pages | 2155-60 |
| PubMed ID | 18519040 | Mgi Jnum | J:201315 |
| Mgi Id | MGI:5512960 | Doi | 10.1016/j.febslet.2008.05.033 |
| Citation | Wu X, et al. (2008) Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B. FEBS Lett 582(15):2155-60 |
| abstractText | Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells. |
