| First Author | Yao CX | Year | 2013 |
| Journal | RNA Biol | Volume | 10 |
| Issue | 4 | Pages | 465-80 |
| PubMed ID | 23558708 | Mgi Jnum | J:242464 |
| Mgi Id | MGI:5905262 | Doi | 10.4161/rna.24370 |
| Citation | Yao CX, et al. (2013) miR-200b targets GATA-4 during cell growth and differentiation. RNA Biol 10(4):465-80 |
| abstractText | GATA-4 is an important transcription factor involved in several developmental processes of the heart, such as cardiac myocyte proliferation, differentiation and survival. The precise mechanisms underlying the regulation of GATA-4 remain unclear, this is especially true for the mechanisms that mediate the post-transcriptional regulation of GATA-4. Here, we demonstrate that miR-200b, a member of the miR-200 family, is a critical regulator of GATA-4. Overexpression of miR-200b leads to the downregulation of GATA-4 mRNA and a decrease in GATA-4 protein levels. Moreover, miR-200b not only inhibits cell growth and differentiation but also reverses the growth response mediated by GATA-4, whereas depletion of miR-200b leads to a slight reversal of the anti-growth response achieved by knocking down endogenous GATA-4. More importantly, the cell cycle-associated gene cyclin D1, which is a downstream target of GATA-4, is also regulated by miR-200b. Thus, miR-200b targets GATA-4 to downregulate the expression of cyclin D1 and myosin heavy chain (MHC), thereby regulating cell growth and differentiation. |
