| First Author | Ueda T | Year | 2009 |
| Journal | Int J Urol | Volume | 16 |
| Issue | 7 | Pages | 639-46 |
| PubMed ID | 19659802 | Mgi Jnum | J:345463 |
| Mgi Id | MGI:7596156 | Doi | 10.1111/j.1442-2042.2009.02325.x |
| Citation | Ueda T, et al. (2009) Unique alternative translation from two open reading frames on Acpin1 mRNA yields an acrosomal protein and a salivary-gland-specific protein. Int J Urol 16(7):639-46 |
| abstractText | OBJECTIVE: To examine the expression profiles of the proteins translated from Acpin1 mRNA in germ cells. METHODS: Northern and western blotting of various tissues and immunohistochemical analysis of germ cells were carried out in a mouse model. RESULTS: ACPIN1 protein was transcribed from the longer, 3' open reading frame (ORF) of Acpin1. An alternative-splicing variant, Acpin1vs, contained only the smaller, 5' ORF of the full-length Acpin1 gene. Its gene product, SAGSIN1, was expressed specifically in salivary glands. Retrotransposed regions of Acpin1 homology were also detected in various chromosomes, and intronless paralogous genes on the X chromosome were expressed in the testis and other tissues. The genomic structure of Acpin1 is highly conserved in mammals. CONCLUSION: The two ORFs on the Acpin1 mRNA are independently translated in differentiated cells. Analysis of gene Acpin1 might clarify the molecular mechanism of spermatogenesis. |
