| First Author | Kitamura T | Year | 2012 |
| Journal | Gene | Volume | 492 |
| Issue | 2 | Pages | 361-7 |
| PubMed ID | 22115574 | Mgi Jnum | J:179382 |
| Mgi Id | MGI:5302142 | Doi | 10.1016/j.gene.2011.11.016 |
| Citation | Kitamura T, et al. (2012) Mouse Wee1 Gene Is Repressed by Kruppel-Like Factor 3 (KLF3) via Interaction with Multiple Upstream Elements. Gene 492(2):361-7 |
| abstractText | Wee1 protein kinase represses CDK1 required for G(2)/M transition. The mouse wee1 promoter contains multiple CACCC-boxes between -306 and +1 that can bind to Kruppel-like factor 3 (KLF3) transcriptional repressor. We found that increasing amounts of intracellular KLF3 decreased the amount of wee1 mRNA. A promoter reporter assay demonstrated that wee1 promoter activity was repressed by KLF3 overexpression. Elimination of the first and fourth CACCC-boxes suppressed KLF3-governed transcriptional repression. A gel-shift assay demonstrated that KLF3 binds to the first, third, and fourth CACCC-boxes with various strengths. Moreover, KLF3 was suggested to interact with the wee1 regulatory region in a physiological condition. Therefore, we concluded that KLF3 is a transcriptional repressor for wee1 gene. In a previous study, we demonstrated that TBP-like protein (TLP) inhibits wee1 promoter function. In this study, we found that the chromosomal wee1 gene is also down-regulated by KLF3. Since KLF3-repressed wee1 promoter function was further inhibited by TLP overexpression regardless of the inhibition degree of KLF3, we propose that TLP and KLF3 repress wee1 promoter independently. |
