| First Author | Fujii T | Year | 2023 |
| Journal | Biochem Biophys Res Commun | Volume | 658 |
| Pages | 55-61 | PubMed ID | 37023615 |
| Mgi Jnum | J:345051 | Mgi Id | MGI:7581109 |
| Doi | 10.1016/j.bbrc.2023.03.066 | Citation | Fujii T, et al. (2023) Activation of mouse Otop3 proton channels by Zn(2). Biochem Biophys Res Commun 658:55-61 |
| abstractText | Otopetrins (Otop1-Otop3) belong to a newly identified family of proton (H(+)) channels activated by extracellular acidification. Here, we found that Zn(2+) activates the mouse Otop3 (mOtop3) proton channels by using electrophysiological patch-clamp techniques. In mOtop3-expressing human embryonic kidney HEK293T cells, a biphasic inward mOtop3 H(+) current comprising a fast transient current followed by a sustained current was observed upon extracellular acidification at pH 5.0. No significant activation of the mOtop3 channel was observed at pH 6.5 and 7.4, but interestingly, Zn(2+) dose-dependently induced a sustained activation of mOtop3 under these pH conditions. Increasing the Zn(2+) concentration had no effect on the reversal potential of the channel currents, suggesting that Zn(2+) does not permeate through the mOtop3. The activation of the mOtop3 channel was specific to Zn(2+) among divalent metal cations. Our findings reveal a novel modulatory mechanism of mOtop3 proton channels by Zn(2+). |
