| First Author | Backer S | Year | 2002 |
| Journal | Neuroscience | Volume | 113 |
| Issue | 4 | Pages | 743-8 |
| PubMed ID | 12182881 | Mgi Jnum | J:80869 |
| Mgi Id | MGI:2447374 | Doi | 10.1016/s0306-4522(02)00221-x |
| Citation | Backer S, et al. (2002) Nr-CAM and TAG-1 are expressed in distinct populations of developing precerebellar and cerebellar neurons. Neuroscience 113(4):743-8 |
| abstractText | Nr-CAM and TAG-1 interact at the floor-plate during the formation of spinal cord commissural projections [Stoeckli, E.T., Landmesser, L.T., Sci. 274 (1995) 1123-1133; Fitzli, D., Stoeckli, E.T., Kunz, S., Siribour, K., Rader, C., Kunz, B., Kozlov, S.V., Buchstaller, A., Lane, R.P., Suter, D.M., Dreyer, W.J., Sonderegger, P., J. Cell. Biol. 149 (2000) 951-968]. We report here the spatio-temporal patterns of expression of these two adhesion molecules during the development of the lower brainstem (medulla and pons) and cerebellum. Nr-CAM and Tag-1 label distinct populations of precerebellar neurons at key steps of their development. Nr-CAM expression starts at E11.5-E12 in the floor-plate, that constitutes an intermediate target during axon outgrowth and nuclear migration of precerebellar neurons. At E13-E14, it is expressed in both floor-plate and inferior olivary nuclei (ION) neurons before being strictly restricted to ION neurons from E15 onwards. Furthermore Nr-CAM, which is widely expressed in the cerebellum during embryonic development, becomes strictly confined to Purkinje and Golgi cells in postnatal cerebellum, suggesting a possible role of Nr-CAM for the maturation or stabilization of the synaptic contacts, in particular between climbing fibers and Purkinje cells. On the other hand, Tag-1 is expressed by migrating neurons that will form the lateral reticular and basilar pontine nuclei. These results emphasize the possibility that TAG-1/Nr-CAM interactions are also involved in the development of the cerebellar system (precerebellar and cerebellar neurons). However, the pattern of cerebellar expression of TAG-1--early migrating Purkinje cells up to E14 and external granular cells--prevents the implication of this adhesion molecule in the organization of extracerebellar projections. |
