| First Author | Kahai S | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 1 | Pages | 233-8 |
| PubMed ID | 19944102 | Mgi Jnum | J:155528 |
| Mgi Id | MGI:4414689 | Doi | 10.1016/j.febslet.2009.11.077 |
| Citation | Kahai S, et al. (2010) Nephronectin promotes osteoblast differentiation via the epidermal growth factor-like repeats. FEBS Lett 584(1):233-8 |
| abstractText | We found that nephronectin was significantly down-regulated by TGF-beta1. To determine the function of nephronectin in osteogenesis, we generated various constructs to produce stable MC3T3-E1 cell lines, expressing and secreting nephronectin protein, including full-length (Npnt), lacking EGF-like repeats (Np-MAM), and lacking RGD and MAM domains (Np-EGF). We demonstrated that nephronectin promotes differentiation during osteoblast differentiation and the EGF-like repeats were essential. Lack of these repeats resulted in inhibiting the change in morphology. Over-expression of nephronectin results in earlier formation of bone nodules than the vector control. ERK activation is essential for nephronectin-induced osteoblast differentiation. |
