| First Author | Yu W | Year | 2023 |
| Journal | Mol Cell Proteomics | Volume | 22 |
| Issue | 6 | Pages | 100564 |
| PubMed ID | 37146716 | Mgi Jnum | J:352418 |
| Mgi Id | MGI:7660987 | Doi | 10.1016/j.mcpro.2023.100564 |
| Citation | Yu W, et al. (2023) STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis. Mol Cell Proteomics 22(6):100564 |
| abstractText | Spermatogenesis defects are important for male infertility; however, the etiology and pathogenesis are still unknown. Herein, we identified two loss-of-function mutations of STK33 in seven individuals with non-obstructive azoospermia. Further functional studies of these frameshift and nonsense mutations revealed that Stk33(-/KI) male mice were sterile, and Stk33(-/KI) sperm were abnormal with defects in the mitochondrial sheath, fibrous sheath, outer dense fiber, and axoneme. Stk33(KI/KI) male mice were subfertile and had oligoasthenozoospermia. Differential phosphoproteomic analysis and in vitro kinase assay identified novel phosphorylation substrates of STK33, fibrous sheath components A-kinase anchoring protein 3 and A-kinase anchoring protein 4, whose expression levels decreased in testis after deletion of Stk33. STK33 regulated the phosphorylation of A-kinase anchoring protein 3/4, affected the assembly of fibrous sheath in the sperm, and played an essential role in spermiogenesis and male infertility. |
