| First Author | Mietton F | Year | 2009 |
| Journal | Mol Cell Biol | Volume | 29 |
| Issue | 1 | Pages | 150-6 |
| PubMed ID | 18936163 | Mgi Jnum | J:219091 |
| Mgi Id | MGI:5619474 | Doi | 10.1128/MCB.00997-08 |
| Citation | Mietton F, et al. (2009) Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. Mol Cell Biol 29(1):150-6 |
| abstractText | We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains approximately 1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability. |
