First Author | Fukunishi Y | Year | 1999 |
Journal | FEBS Lett | Volume | 464 |
Issue | 3 | Pages | 129-32 |
PubMed ID | 10618491 | Mgi Jnum | J:59260 |
Mgi Id | MGI:1351254 | Doi | 10.1016/s0014-5793(99)01696-8 |
Citation | Fukunishi Y, et al. (1999) Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database. FEBS Lett 464(3):129-32 |
abstractText | We propose a prediction method for human full-length cDNA by comparing sequence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is selected by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA. The accuracy of the predicted human full-length coding region is 83.3%, and the false positive rate is 16.7%. Five human full-length proteins out of 20 proteins are correctly predicted. |