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Publication : Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database.

First Author  Fukunishi Y Year  1999
Journal  FEBS Lett Volume  464
Issue  3 Pages  129-32
PubMed ID  10618491 Mgi Jnum  J:59260
Mgi Id  MGI:1351254 Doi  10.1016/s0014-5793(99)01696-8
Citation  Fukunishi Y, et al. (1999) Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database. FEBS Lett 464(3):129-32
abstractText  We propose a prediction method for human full-length cDNA by comparing sequence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is selected by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA. The accuracy of the predicted human full-length coding region is 83.3%, and the false positive rate is 16.7%. Five human full-length proteins out of 20 proteins are correctly predicted.
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