| First Author | Bandyopadhyay A | Year | 2000 |
| Journal | Mol Biol Cell | Volume | 11 |
| Issue | 11 | Pages | 4005-18 |
| PubMed ID | 11071923 | Mgi Jnum | J:66269 |
| Mgi Id | MGI:1928208 | Doi | 10.1091/mbc.11.11.4005 |
| Citation | Bandyopadhyay A, et al. (2000) Fission yeast int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation. Mol Biol Cell 11(11):4005-18 |
| abstractText | Mammalian INT6 protein has been considered to be a subunit of the eukaryotic translation initiation factor, eIF3. The Int6 locus is also known as a common integration site of mouse mammary tumor virus (MMTV). However, the function of Int6 in translation initiation and the mechanism of Int6-mediated tumor induction are yet to be explored. In this study, the fission yeast, Schizosaccharomyces pombe, int6(+), which is 43% identical to the mammalian counterpart, was deleted. Despite the evidence that the majority of Int6 protein was associated with 40S particles in this organism, strains lacking int6(+) (Deltaint6) were viable and showed only moderate inhibition in the rate of in vivo global protein synthesis. Polysome profile analysis showed no apparent defects in translation initiation. Deltaint6 exhibited a hypersensitivity to caffeine, which could be suppressed by the addition of sorbitol to the growth medium. This and other phenotypes would imply that int6(+) is required for the integrity of cell membrane. In meiosis, Deltaint6 produced incomplete tetrads frequently. High dosage expression of a truncated mutant of int6(+) conferred a hypersensitivity to caffeine, but did not cause the defect in meiosis. A possible link between the function of int6(+) and the Deltaint6-phenotypes is discussed. |
