| First Author | Shinohara T | Year | 1982 |
| Journal | Proc Natl Acad Sci U S A | Volume | 79 |
| Issue | 9 | Pages | 2783-7 |
| PubMed ID | 6953430 | Mgi Jnum | J:6796 |
| Mgi Id | MGI:55268 | Doi | 10.1073/pnas.79.9.2783 |
| Citation | Shinohara T, et al. (1982) Multiple gamma-crystallins of the mouse lens: fractionation of mRNAs by cDNA cloning. Proc Natl Acad Sci U S A 79(9):2783-7 |
| abstractText | cDNAs made from polyadenylylated RNAs of the mouse lens were cloned by the G.C tailing procedure in the bacterial plasmid pBR322. Four recombinant DNAs containing gamma-crystallin sequences were identified by hybrid selection and translation. Sequence analysis of the in vivo-labeled gamma-crystallin polypeptides that cofocused isoelectrically with the hybrid-selected translation products established that the four cloned cDNAs were derived from mRNAs encoding gamma-crystallin polypeptides with similar NH2 termini. The cDNA clones had different restriction maps and could discriminate among the different gamma-crystallin mRNAs under stringent hybridization conditions. Under relaxed hybridization conditions, the cDNA clones cross-hybridized with all gamma-crystallin mRNAs, and even slightly with beta-crystallin mRNAs, as judged by in vitro translation. RNA blot hybridization showed that the mouse lens gamma-crystallin mRNAs are 840 +/- 100 nucleotides long. These data indicate that there are at least four similar gamma-crystallin mRNAs and suggest (but do not establish) the existence of a closely related family of gamma-crystallin genes. |
